The isolation of a new warfarin-sensitive protein from bovine plasma.

نویسندگان

  • C V Prowse
  • M P Esnouf
چکیده

immobilized lipoamide dehydrogenase in 30 % (v/v) dioxan. The rate constants for inactivation in dioxan, like those for thermal inactivation at 90°C, increase with increasing distance from the matrix and eventually approach that for the inactivation of the native enzyme in 30% (v/v) dioxan, i.e. 0.01 min-'. These data can be rationalized by the fact that the hydrophilic Sepharose matrix probably holds the enzyme in a rigid conformation, and thus the nearer the enzyme is to the matrix backbone, the greater its stability. These observations contrast markedly with the destabilization of proteins bound to the hydrophobic matrix polystyrene reported by Manecke (1962).

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

INVESTIGATIONS ON THE DRUG-PROTEIN IN TERAC TION OF CERTAIN NEW POTENTIAL LOCAL ANAESTHETICS

Generally, plasma proteins owe their binding capacity to the presence of aminoacid units which enter into intra- and intermolecular hydrophobic bonding with a diverse range of endo- and exogenous chemical substances. The intermolecular interactions between the hydrophobic areas of drug molecules and those of plasma proteins play an important role in drug-macromolecular complex formation and...

متن کامل

ISOLATION OF MYELIN BASIC PROTEIN AND DETECTION OF I TS IMMUNOL\'OGICAL PROPERTIES

Multiple sclerosis (MS) is an inflammatory disease of the central nervous system of presumed autoimmune etiology. One of the best animal models of demyelinating diseases is experimental autoimmune encephalomyelitis (EAE), which can be induced in a variety of animals by injection of a target antigen such as myelin basic protein (MBP). The immune responses against the target amino acids caus...

متن کامل

Isolation and purification of albumin from human plasma by direct and combined approach of ion-exchange chromatography and comparison of the final products quality obtained by both methods

Background: Due to multiple roles of albumin in the body, injection of its medicinal product as one of the therapeutic or management strategies under conditions such as severe bleeding, burns, liver failure, and neonatal hemolytic diseases is on the physicians' agenda. Considering that albumin is the most abundant plasma protein, designing an appropriate method to purify it is highly important....

متن کامل

جداسازی و خالص سازی لاکتوفرین از شیر گاو

Background and purpose: Lactoferrin is a multifunctional protein, which is useful for clinical and commercial applications. Recently it has been shown that ingestion of Lactoferrin has advantages including: immune system modulation, anti-microbial activity and antioxidant in neonates and adults in both animals and humans. The present study has been conducted with the purpose of isolation and op...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Biochemical Society transactions

دوره 5 1  شماره 

صفحات  -

تاریخ انتشار 1977